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KMID : 0903519900330040287
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1990 Volume.33 No. 4 p.287 ~ p.292
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Bowman - Birk type proteinase inhibitors from soybean : Isolation and partial characterization
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Abstract
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Eight(I through VIII) of Bowman-Birk proteinase inhibitors have been isolated from soybean with DEAF-Sephadex A-50. Inhibitor VII was a typical BBTI, showing high cysteine content(17%/mole) and low trypsin to chymotrypsin inhibiting activity(TIA/CIA=1.0) with the independent reactive site to tech enzyme. Dissociation constant of trypsin-BBTI and chymotrypsin-BBTI complexes were 9.17 × 10^(-9)M and 5.14 × 10^(-9)M, respectively. Inhibitor VII was extremely heat stable. Six hours hit treatment at 100℃ caused only 50% decrease in it´s original inhibiting activity. Except inhibitor III, 6 other isoinhibitors differed from a typical BBTI in TIA/CIA values, ranging from 3 to 29.
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